Abstract: Pressure resistance is an essential characteristic of microspheres which has applications in various fields but the manual measurement of it is laborious and inaccurate. The method of automatic measurement using image detection to discriminate microspheres failures is proposed. Gradient Hough transform is used to localize microspheres. Contours are extracted via Canny edge detection and environment disturbances are also alleviated; finally Hu invariant moments are employed to implement image matching. A customized experiment platform was established and experiments involving glass and polymer microspheres with different diameters were conducted to examine the accuracy and robustness of the method. Nearly 100% success rate for discrimination of failed microspheres was achieved and the pressures at which microspheres failed were recorded with little delay.