Detection of spike protein of SARS-CoV-2 by surface enhanced Raman spectroscopy
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摘要:
开发具有高灵敏度、高准确性的新型冠状病毒(SARS-CoV-2)快速检测技术对疫情防控具有重要作用。本文利用表面增强拉曼光谱(SERS)技术对人体唾液中的痕量SARS-CoV-2病毒刺突蛋白(S蛋白)进行了检测。结果表明,含S蛋白的唾液样本与原始唾液样本的拉曼光谱具有显著区别,含S蛋白的唾液样本谱图中可清晰观察到属于S蛋白的拉曼谱线。该结果为后续SERS技术在SARS-CoV-2病毒快速检测方面的应用奠定了坚实基础。
Abstract:The development of rapid detection technique with high sensitivity and high veracity for SARS-CoV-2 is of great importance for the prevention and control of epidemic. In this work, the trace spike protein (S protein) of SARS-CoV-2 in human saliva was detected by surface enhanced Raman spectroscopy (SERS) technique. Significant differences can be found between the Raman spectra of the S protein stained saliva sample and the primitive saliva sample. These results will lay the foundation for the future rapid detection of SARS-CoV-2 by SERS technique.
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Key words:
- surface enhanced Raman spectroscopy /
- SARS-CoV-2 /
- spike protein /
- rapid detection
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