Three-dimensional reconstruction system for transparent samples based on phase retrieval

Cao Haoyue; Zhao Chen; Liu Jing; Peng He; Zhou Yang; Yang Shuwei; Ma Yue; Lu Yibing

doi:10.11884/HPLPB202436.240128

Volume 36 Issue 9

Aug. 2024

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Article Navigation > High Power Laser and Particle Beams > 2024 > 36(9): 091003

Cao Haoyue, Zhao Chen, Liu Jing, et al. Three-dimensional reconstruction system for transparent samples based on phase retrieval[J]. High Power Laser and Particle Beams, 2024, 36: 091003. doi: 10.11884/HPLPB202436.240128

Citation:

Cao Haoyue, Zhao Chen, Liu Jing, et al. Three-dimensional reconstruction system for transparent samples based on phase retrieval[J]. High Power Laser and Particle Beams, 2024, 36: 091003. doi: 10.11884/HPLPB202436.240128

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Three-dimensional reconstruction system for transparent samples based on phase retrieval

doi: 10.11884/HPLPB202436.240128

1.
School of Electronic Information Engineering, Xi’an Technological University, Xi’an 710021, China
2.
College of Medicine, Xi 'an International University, Xi 'an 710077, China
3.
School of Intelligent Science and Engineering, Xi 'an Peihua University, Xi 'an 710125, China

Received Date: 2024-04-16
Accepted Date: 2024-07-01
Rev Recd Date: 2024-07-01

Available Online: 2024-07-09

Publish Date: 2024-08-16

Abstract

Abstract

In response to the difficulties posed by traditional microscopy imaging techniques in capturing the structure and thickness of colorless transparent samples, we have designed a miniature three-dimensional reconstruction system for such samples. This innovative system, breaking away from traditional optical structures, performs phase retrieval on transparent samples to achieve three-dimensional reconstruction. It requires only light carrying sample information, which is then bifurcated by a spectroscope and captured by a stereo camera. Constructed using 3D printing technology, the compact system measures just 110 mm×110 mm×60 mm, offering a cost-effective solution that is also compatible with traditional microscopy imaging equipment. It incorporates autofocus and field of view correction algorithms, which, by collecting one over-focused and one under-focused image, solve the transport intensity equation to enable phase retrieval and hence the three-dimensional reconstruction of transparent samples. Test results have shown that the system can achieve an imaging resolution of 2.46 μm under a 10× objective lens, and the phase recovery accuracy can also meet the basic requirements. Furthermore, the successful three-dimensional reconstruction of blood cells and scratches on microscope slides validates the system's feasibility and practicality.
- microscopy imaging,
- three-dimensional reconstruction,
- 3D printing,
- transport intensity equation,
- phase retrieval

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References(15)

References

[1]	左超, 陈钱. 计算光学成像: 何来, 何处, 何去, 何从?[J]. 红外与激光工程, 2022, 51:20220110 doi: 10.3788/IRLA20220110 Zuo Chao, Chen Qian. Computational optical imaging: an overview[J]. Infrared and Laser Engineering, 2022, 51: 20220110 doi: 10.3788/IRLA20220110
[2]	Jiang Fuda, Zhang Chonglei. High accuracy quantitative phase imaging based on transport-of-intensity equation[J]. Optics and Lasers in Engineering, 2023, 169: 107700. doi: 10.1016/j.optlaseng.2023.107700
[3]	Park Y, Depeursinge C, Popescu G. Quantitative phase imaging in biomedicine[J]. Nature Photonics, 2018, 12(10): 578-589. doi: 10.1038/s41566-018-0253-x
[4]	Zuo Chao, Li Jiaji, Sun Jiasong, et al. Transport of intensity equation: a tutorial[J]. Optics and Lasers in Engineering, 2020, 135: 106187. doi: 10.1016/j.optlaseng.2020.106187
[5]	何璇. 明场、暗场、相衬的多模显微镜成像技术研究[D]. 成都: 电子科技大学, 2017 He Xuan. Research on multi-mode microscopy imaging technology of brightfield, darkfield, phase contrast[D]. Chengdu: University of Electronic Science and Technology of China, 2017
[6]	Trattner S, Kashdan E, Feigin M, et al. Image formation of thick three-dimensional objects in differential-interference-contrast microscopy[J]. Journal of the Optical Society of America A, 2014, 31(5): 968-980. doi: 10.1364/JOSAA.31.000968
[7]	左超, 陈钱, 孙佳嵩, 等. 基于光强传输方程的非干涉相位恢复与定量相位显微成像: 文献综述与最新进展[J]. 中国激光, 2016, 43:0609002 doi: 10.3788/CJL201643.0609002 Zuo Chao, Chen Qian, Sun Jiasong, et al. Non-interferometric phase retrieval and quantitative phase microscopy based on transport of intensity equation: a review[J]. Chinese Journal of Lasers, 2016, 43: 0609002 doi: 10.3788/CJL201643.0609002
[8]	桂博瀚, 李常伟. 基于波面分割及多平面相位恢复的定量相位成像技术[J]. 光学学报, 2023, 43:1411002 doi: 10.3788/AOS230451 Gui Bohan, Li Changwei. Quantitative phase imaging technology based on wavefront segmentation and multiplane phase retrieval[J]. Acta Optica Sinica, 2023, 43: 1411002 doi: 10.3788/AOS230451
[9]	张赵. 基于光强传输方程的相位恢复与多模式成像研究[D]. 南京: 南京理工大学, 2017 Zhang Zhao. Phase retrieval and multi-mode imaging based on light intensity transfer equation[D]. Nanjing: University of Nanjing University of Science and Technology, 2017
[10]	Cheng Hong, Wang Jincheng, Gao Yaoli, et al. Phase unwrapping based on transport-of-intensity equation with two wavelengths[J]. Optical Engineering, 2019, 58: 054103.
[11]	Grant S D, Richford K, Burdett H L, et al. Low-cost, open-access quantitative phase imaging of algal cells using the transport of intensity equation[J]. Royal Society Open Science, 2020, 7: 191921. doi: 10.1098/rsos.191921
[12]	Chen Chao, Lu Y Yunan, Huang Huachuan, et al. PhaseRMiC: phase real-time microscope camera for live cell imaging[J]. Biomedical Optics Express, 2021, 12(8): 5261-5271. doi: 10.1364/BOE.430115
[13]	Liu Cheng, Wang Shouyu, Veetil S P. Computational optical phase imaging[M]. Singapore: Springer, 2022.
[14]	Carney S, Khoo T C, Sheikhsofla A, et al. Quantitative phase imaging comparison of digital holographic microscopy and transport of intensity equation phase through simultaneous measurements of live cells[J]. Optics and Lasers in Engineering, 2023, 166: 107581. doi: 10.1016/j.optlaseng.2023.107581
[15]	Wang Shouyu, Huang Huachuan, Sun Aihui, et al. Dual-view transport of intensity phase imaging devices for quantitative phase microscopy applications[J]. Sensors & Diagnostics, 2024, 3(3): 381-394.