In order to systemically investigate the morphology and microstructure induced in fused silica by UV (355 nm) laser pulses at high fluence, a suite of microscopic tools have been used, such as Nomarski optics microscope,AFM and SEM. Two types of damage are observed on the input surface of fused silica, that is, micro-pits and star-like cracks with diameters of 0.8~2.5 μm and 1.0~5.5 μm, respectively. Correspondingly, there are three types of damage on the output surface, namely micro-pits,shell-like damage and craters with diameters of 0.48~1.33 μm,4~20 μm and 12~30 μm, respectively. By comparing morphology of fused silica and laser fluence, several assumptions have been given to understand the damage initiation mechanism,especially for 1 μm scale laser initialed damage.