Abstract: To realize the calculation and analysis for the neutron activation character of the 14 MeV D-T rotary target chamber, a new approach coupling the self-developed activation code BURNDOT with the Monte Carlo particle transport calculation code MCNP and the MCNP Automatic Modeling (MCAM) was developed, which integrates the functions of neutron transport, activation of materials and decay gamma transport.The influence of the factors including materials, cells and main activated nuclides on activation character of rotary target chamber at different time after the irradiation was analyzed in detail.The results show that the time about 68 hafter neutron radiation acts as a boundary, before it the copper material is the major contributor for activity instead of the 316 stainless steel after it.For the former, the delayedγdose rate up to maximum is due to 62 Cu and 64 Cu, and for the latter, the delayedγdose rate is influenced by the long life-time nuclides such as 55 Fe, 57 Co and 54 Mn and it is under the level of 10μSv/h.In addition, the results were compared with the data of the European activation code FISPACT-2007.