Abstract: To explore laser induced thermal injure in skin tissue, 1064 nm laser induced thermal injure in mice skin with different laser duration was studied by experimental and theoretical analysis methods. Dermoscope images and optical coherence tomography images were used to assess the degree of thermal injure in biological skin tissue. Arrhenius thermal injure equation was used to calculate thermal damage parameters and establish a laser induced thermal injure model of skin tissue, then compared with experimental results. The results show that under the 1064 nm laser irradiation with 30 W/mm² power density, there was reversible damage in the mouse skin tissue within 0 to 100 ms laser duration; the mice skin tissue appeared edema and thermal coagulation injure within 150 to 280 ms laser duration; during the laser duration of 280 to 550 ms, the epidermis of the mice skin was vaporized, eschars appeared around the damage spot, and the dermis was degenerated. Over 660 ms laser duration, the epidermis and dermis of the mice skin were vaporized, tissue fluid was exuded from the wound and the subcutaneous tissue was degenerated. The theoretical analysis is consistent with the experimental results. The established thermal injury model can verify the degree of thermal injury in mice skin.