Abstract: Multichannel high-speed data acquisition software is facing the following difficulties: complexity in setup and management of signal multichannel; The concurrent transmission of data requires high stability and instantaneous transmission speed; High-speed post-processing and high-speed waveform display are required for large amount of measurement data; The test software must be stable and reliable to meet the important requirements of experimental data. The software uses modular graphical design and SCPI to complete channel setup. The transmission speed of data uploaded simultaneously by 75 oscilloscopes was tested. The differences between LabView waveform display and GDI+ waveform drawing are compared. Data acquisition function in case of software failure is designed. It is shown that the data acquisition software can manage 75 oscilloscopes and upload, process, display and store 300 channels of data. 100 kpts memory 300 channel data upload time is 9.7s, 300 channel waveform refresh time is 0.91 s.