Abstract: Gas-liquid separation is the key to the success of an aerosol-type singlet oxygen generator. To solve the problem of gas-liquid separation, the idea of droplet size-controlled centrifugal separation was proposed that size-controlled droplets are produced by using an atomization technique and then during the reaction of the droplets with gas flow the gas-liquid separation is simultaneously done by a centrifugal force which is determined by the sizes of the droplets and generated by a set of high-speed rotating blades. To verify this idea, a twisted flow aerosol singlet oxygen generator (TFA-SOG) based on this idea was built and investigated through simulation and experiment. The investigation shows that the simulated gas-liquid separation efficiencies are consistent with the experimental ones and the idea is feasible; the emphasis on further development of TFA-SOGs should be put on the droplet size-controlled atomization technique.