Abstract: This paper proposes a layout structure of the pulsed power generator based on the bipolar linear transformer driver, achieving flexible stacking of the bipolar linear transformer driver modules. The conduction time of the metal-oxide-semiconductor field effect transistor is regulated by adjusting the voltage of the driver circuit, enabling precise control over the rise time of the pulsed voltage. An integrated core-copper pillar structure and a reverse overshoot discharge circuit are introduced in the linear transformer driver structure, which optimizes the electromagnetic compatibility and reduces the reverse overshoot at the tail of the pulsed waveform. The developed bipolar linear transformer driver device can stably output ±5 kV pulsed voltage with 1 kHz frequency and 1 μs pulse width. The rise time of the pulsed voltage is continuously adjustable from 30 ns to 100 ns. The irreversible electroporation experiments on cells are carried out using the bipolar linear transformer driver.