Abstract: The experimental results of the stable high-order harmonically mode-locked pulses generation, amplification and compression in Yb3+-doped fiber laser system were reported. Using nonlinear polarization rotation(NPR) effect, the artifical saturable absorber was generated and the stable high-order harmonically mode-locked pulses were generated from Yb3+-doped fiber ring laser. The characteristics of Yb3+-doped mode-locked fiber laser were analyzed theoretically. Three different ways were found, with which high-order harmonically mode-locked pulses were generated from Yb3+-doped mode-locked fiber laser. The stable 4th harmonically mode-locked (107.2 MHz repetition rate) pulses were amplified to an average power of 100 mW and pulse width of 22.8 ps through an amplifier with 1 m high concentrati