Experimental study on Escherichia coli treatment by pulsed xenon lamp
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摘要: 为优化脉冲氙灯灭菌装置性能，基于自主研发的微秒脉冲电源系统，研究了不同规格的定制氙灯及其光谱范围对灭菌效果的影响。结果表明：弧长50 mm、气压50 kPa的氙灯的紫外-可见部分光谱中，UV波段占38.5%，UVC波段占17.6%。增大弧长、减小气压均可提高光谱强度，后者还可提高UV比例。弧长100 mm，气压50 kPa的氙灯在放电能量为20 J时，处理3 s可使覆盖范围内的大肠杆菌基本全部失去活性。灭菌效率与灯管弧长、放电能量呈正相关，与灯管气压呈负相关。氙灯辐射光的不同波段均有灭菌作用，UV波段灭菌对数值占87.7%，小于280 nm波段占64.6%。原子力显微镜结果显示，脉冲氙灯可以改变细菌菌体形貌和力学性质，使菌体萎缩，增大表面粗糙度、菌体弹性和粘附力。Abstract: To optimize the performance of the pulsed xenon lamp sterilization device, the influence of spectral range and specifications of lamps on the sterilization effect is studied based on a self-developed high-energy microsecond pulse power supply and xenon lamps with different specifications. The results show that in the UV-visible spectrum of a xenon lamp with an arc length of 50 mm and a pressure of 50 kPa, the UV accounts for 38.5% and the UVC accounts for 17.6%. Increasing the arc length and decreasing the pressure can both increase the spectral intensity, and the latter can also increase the ratio of UV. The xenon lamp with an arc length of 100 mm and a pressure of 50 kPa can basically inactivate all Escherichia coli in 3 seconds with a discharge energy of 20 J. The sterilization rate is positively correlated with arc length and discharge energy of the lamp, negatively correlated with pressure. The all bands of xenon lamp radiation have sterilization effects, with UV accounting for 87.7% and the wavelength band less than 280 nm accounting for 64.6%. The AFM images show that pulsed xenon lamp changed the morphology and mechanical properties of Escherichia coli, hence the bacteria shrank, their surface roughness, elasticity, and adhesion increased.
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Key words:
- pulsed xenon lamp /
- surface sterilization /
- pulse discharge /
- intense pulsed light /
- Escherichia coli
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图 1 脉冲氙灯灭菌实验装置示意图
Figure 1. Schematic diagram of pulsed xenon lamp sterilization experimental setup
图 4 不同条件下脉冲氙灯的光谱分布
Figure 4. Spectral distribution of pulsed xenon lamp under different conditions
图 5 不同弧长脉冲氙灯的灭菌效果
Figure 5. Sterilization effect of pulsed xenon lamp with different arc lengths
图 6 不同气压脉冲氙灯的灭菌效果
Figure 6. Sterilization effect of pulsed xenon lamp with different gas pressures
图 7 不同放电能量下脉冲氙灯的灭菌效果
Figure 7. Sterilization effect of pulsed xenon lamp under different discharge energies
图 8 脉冲氙灯不同子波段辐射的灭菌效果
Figure 8. Sterilization effect of pulsed xenon lamp under different sub-bands
图 9 脉冲氙灯不同子波段辐射的灭菌对数值
Figure 9. Sub-band sterilization logarithm value of pulsed xenon lamp
图 10 处理前后大肠杆菌的3D图、高度图与剖面分析图
Figure 10. The 3D images, height images and corresponding section analysis images of Escherichia coil before and after treatment
图 11 处理前后大肠杆菌的相图与相位剖面分析图
Figure 11. The phase images and corresponding section analysis images of Escherichia coli before and after treatment
图 12 处理前后大肠杆菌的力曲线图
Figure 12. The force curves of Escherichia coli before and after treatment
表 1 不同规格脉冲氙灯的尺寸参数
Table 1. Dimensional parameters of different pulsed xenon lamps
total length/mm arc length/mm gas pressure/kPa outer diameter/mm 110 50 25 7.0 110 50 50 7.0 135 75 25 7.0 135 75 50 7.0 160 100 25 7.0 160 100 50 7.0 
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表 2 处理前后大肠杆菌的粗糙度
Table 2. The roughness of Escherichia coli before and after treatment
No. before treatment after treatment Ra Rq Ra Rq 1 17.5 21.4 19.8 24.5 2 18.1 22.3 24.8 31.0 3 16.3 20.5 19.9 24.7 4 16.4 20.1 18.5 23.2 5 19.2 25.7 22.5 29.7 6 15.5 18.7 22.0 31.4 7 19.0 23.6 25.4 29.3 8 16.7 20.4 20.1 25.7 9 17.6 22.4 22.3 28.3 10 18.6 22.5 19.6 24.2 average 17.5 21.8 21.5 27.2 standard deviation 1.2 1.9 2.2 2.9
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