Abstract: To observe whether the high-power electron accelerator achieves the design index performance and disinfection effect, the performance of the high-power electron accelerator using the methods and procedures specified in the standard was tested and evaluated, and the inactivation effect of the electron beam output from the electron accelerator on microorganisms such as Staphylococcus aureus, Escherichia coli, Bacillus cereus, coronavirus, was explored. At the same time, examined how different material loads affected the inactivation efficiency. The high-power electron accelerator, can output a high-energy electron beam with an energy of 10.27 MeV and a power of 25 kW and it was found to have a certain level of penetration capability for surrogate materials. The electron beam achieves a disinfection effect of 3 log reductions or higher against microorganisms such as Staphylococcus aureus, Escherichia coli, Bacillus cereus, and coronaviruses. After penetrating a certain thickness of load material, the electron beam can still achieve a disinfection effect of 3 log reductions or higher against Staphylococcus aureus, Escherichia coli, Bacillus cereus, and Bacillus subtilis (black variant) spores. The designed electron accelerator meets the performance specifications and has a certain level of penetration capacity, effectively achieving the required disinfection effect against the studied microorganisms.